Protein and amino acid content determined using an automated Kjeldahl determination

The mushroom is a famous edible fungus, belonging to the genus Agaricaceae of the genus Basidiomycetes. It is rich in nutritive value and contains many kinds of fungi. Functional proteins have anti-cancer, anti-allergic, and improve the body's ability to kill rabbits, lower cholesterol and so on. The contents of protein and total amino acids in wild velutine mushroom were measured for the first time by using automatic Kjeldahl nitrogen analyzer and automatic amino acid analyzer to provide the basis for the development and utilization of wild velutine mushroom.

Accurately weigh 0.2g of wild needle mushroom sample into automatic Kjeldahl digestion tube and add 49 potassium sulfate pentahydrate and potassium sulfate mixture (copper sulfate pentahydrate: potassium sulfate-1'6), concentrated sulfuric acid 10mL,}X Oxygen and water 1mL mix,) The digestive tube into the electric furnace, slowly heated to boiling, until the solution becomes clear and green after heating for 2h, digestion of the sample on the machine analysis.

According to the results obtained by automatic Kjeldahl analysis, the content of protein in the sample was calculated (Nx4.38)}'}} The average protein content of wild velutine mushroom was 31.171m, leaving 100mg; the content of protein in the three samples was sample}} }8.167mb/100m}>Sample 1X29.165mg/100m}>Sample 2 (26.181 mb/100mg), indicating that there is a difference in the protein content of wild enoki mushrooms grown on different substrates; in comparison, the growth on eucalyptus The content of protein in Flammulina velutipes is much higher than that of Flammulina velutipes grown on the deciduous tree. The content of protein in the two Flammulina velutipes samples grown in the tree was below average.

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